electroporation cuvettes (Bio-Rad)
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electroporation cuvettes
Electroporation Cuvettes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 866 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/electroporation+cuvettes/Gene+Pulser+%2FMicroPulser+Electroporation+Cuvettes/pmc12887785-63-21-23
Average 96 stars, based on 866 article reviews
Electroporation Cuvettes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 866 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/electroporation+cuvettes/Gene+Pulser+%2FMicroPulser+Electroporation+Cuvettes/pmc12887785-63-21-23
Average 96 stars, based on 866 article reviews
electroporation cuvettes - by Bioz Stars,
2026-09
96/100 stars
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1) Product Images from "M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage"
Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage
Journal: Bioactive Materials
doi: 10.1016/j.bioactmat.2026.01.047
Figure Legend Snippet: Schematic illustration of the preparation of M2-exo@HI and its mediated therapeutic mechanisms and signaling pathways. (a) RAW264.7 macrophages were polarized to M2 phenotype using DEX, followed by M2-exo isolation from cell supernatant via differential centrifugation. M2-exo@HI was prepared through encapsulating HI into M2-exo by electroporation. (b) M2-exo@HI crossed the BBB and localized to microglia in the hemorrhagic brain, delivering the HI plasmid into the nucleus. This prompted expression and secretion of Hp and IL-10 by microglia. The released Hp inhibited Hb toxicity by binding to Hb. IL-10 shifted microglial polarization from the pro-inflammatory M1 phenotype toward the reparative M2 phenotype, removing hematoma by engulfing erythrocyte and Hb-Hp complex, and decreasing pro-inflammatory cytokine levels—collectively enhancing neuroprotection and BBB repair. These beneficial outcomes were linked to the inhibition of the IL-17 and NF-κB signaling pathways and activation of the PI3K-Akt and ferroptosis pathways.
Techniques Used: Protein-Protein interactions, Isolation, Centrifugation, Electroporation, Plasmid Preparation, Expressing, Binding Assay, Inhibition, Activation Assay
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Electroporation:Article Title: Development and application of a barcoded rabies viral tracing method for mapping brain-wide inputs to single neurons Article Snippet: .. 4 mm Article Title: BioBloom, a method for barcoded saturation mutagenesis of an entire bacterial genome Article Snippet: Both insert and vector fragments were cleaned/concentrated using magnetic DNA-binding beads (Sergi Lab Supplies), and assembled using NEBridge Golden Gate Assembly Mix (Bsal-HF-v2, New England Biolabs (NEB)) following manufacturer recommendations, except that roughly double the recommended DNA per reaction volume was used, the reaction was run for 12 hours total, and three 400uL reactions were used, to maximize the output CFU following transformation. .. After cleaning and concentrating the resulting assemblies they were electroporated into NEB 10-beta electrocompetent E. coli (NEB). targeting 0.5-lug DNA per 100uL electroporation in 2mm Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage Article Snippet: .. M2-exo (100 μL, 1 mg/mL), HI plasmid (circular pBudCE4.1, 100 μg, TsingkeBiotechnology., Ltd.), and 100 μL PBS were added to the Article Title: One-step approach producing barcoded rabies virus with optimized diversity Article Snippet: .. 4 mm Article Title: One-step approach producing barcoded rabies virus with optimized diversity Article Snippet: .. Then, 80 μL of suspension was transferred into each of 15 Article Title: Evolutionary algorithms accelerate de novo design of potent Nectin-4-specific cancer biologics Article Snippet: The obtained oligo pool from Twist Biosciences was amplified with PCR (primers: primers pool-amplification_fwd, primers pool-amplification_rev) for 20 cycles. .. After ligation, the library was transformed into Endura electrocompetent E. coli (LGC Biosearch Technologies, #60242-1) by electroporation in 0.1 cm Article Title: Pulsed electric fields-mediated inactivation of foodborne pathogens in bovine colostrum Article Snippet: .. Subsequently, 400 μL of the inoculated colostrum was added to Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage Article Snippet: .. M2-exo (100 μL, 1 mg/mL), HI plasmid (circular pBudCE4.1, 100 μg, TsingkeBiotechnology., Ltd.), and 100 μL PBS were added to the Plasmid Preparation:Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage Article Snippet: .. M2-exo (100 μL, 1 mg/mL), HI plasmid (circular pBudCE4.1, 100 μg, TsingkeBiotechnology., Ltd.), and 100 μL PBS were added to the Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage Article Snippet: .. M2-exo (100 μL, 1 mg/mL), HI plasmid (circular pBudCE4.1, 100 μg, TsingkeBiotechnology., Ltd.), and 100 μL PBS were added to the Suspension:Article Title: One-step approach producing barcoded rabies virus with optimized diversity Article Snippet: .. Then, 80 μL of suspension was transferred into each of 15 Ligation:Article Title: Evolutionary algorithms accelerate de novo design of potent Nectin-4-specific cancer biologics Article Snippet: The obtained oligo pool from Twist Biosciences was amplified with PCR (primers: primers pool-amplification_fwd, primers pool-amplification_rev) for 20 cycles. .. After ligation, the library was transformed into Endura electrocompetent E. coli (LGC Biosearch Technologies, #60242-1) by electroporation in 0.1 cm Transformation Assay:Article Title: Evolutionary algorithms accelerate de novo design of potent Nectin-4-specific cancer biologics Article Snippet: The obtained oligo pool from Twist Biosciences was amplified with PCR (primers: primers pool-amplification_fwd, primers pool-amplification_rev) for 20 cycles. .. After ligation, the library was transformed into Endura electrocompetent E. coli (LGC Biosearch Technologies, #60242-1) by electroporation in 0.1 cm Bacteria:Article Title: Pulsed electric fields-mediated inactivation of foodborne pathogens in bovine colostrum Article Snippet: .. Subsequently, 400 μL of the inoculated colostrum was added to |